Adaptive dynamic artificial poly-ligand targeting (ADAPT) enables plasma-based exosome profiling with potential diagnostic utility (Late-Breaker)


Valeriy Domenyuk, Zhenyu Zhong, Adam Stark, Nianqing Xiao, Heather O’Neill, Jie Wang, Xixi Wei; Teresa Tinder, Janet Duncan, Andrew Hunter, Mark R. Miglarese, Joachim Schorr, David Halbert, John Quackenbush, George Poste, Günter Mayer, Michael Famulok, and David Spetzler


Single stranded DNA (ssDNA) libraries consisting of several trillion oligodeoxynucleotides (ODNs) can adopt a nearly infinite number of three-dimensional structures. These structures can potentially bind any biomolecule and can be screened for specificity toward important biomarkers by employing suitable enrichment schemes. Since no prior knowledge on the binding partner is required, massively parallel biomarker identification is possible even on complex matrices like biological fluids and across a wide range of biological conditions. Here we present Adaptive Dynamic Artificial Poly-ligand Targeting (ADAPT) as a platform for biomarker and target discovery. We employed ADAPT for the molecular profiling of exosome-associated proteins in small volume plasma samples from women with breast cancer and healthy donors.

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